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. 2023 Apr 3;61(1):621–629. doi: 10.1080/13880209.2023.2189908

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© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — Sch B regulates NCOA4-mediated ferritinophagy in activated HSCs. (a) Immunofluorescence staining analysis of the localization of NCOA4 and LC3B in liver tissue. Nuclear was shown by DAPI. Scale bar, 10 μm (n = 3). (b, c) Western blot analyses of NCOA4 and LC3B in LX2 cells (n = 3). Data were expressed as mean ± SD. Significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control. (d) Immunofluorescence staining analysis of the localization of NCOA4 and LC3B in LX2 cells. Nuclear was shown by DAPI. Scale bar, 7.5 μm (n = 3). (e) Western blot analyses of FTH1 in LX2 cells (n = 3). Data were expressed as mean ± SD. Significance: **P < 0.01, ***P < 0.001 vs. Control. (f) Co-IP analysis of the interaction between FTH1 and NCOA4.