Table 2.

List of all most significant association between DNA methylation and each sex hormone and SHBG at each sampling time point, respectively.

Hormone	CpG	Time point	P.value	FDR	Gene	Location	Median methylation value (1st Q. − 3rd Q.)	Effect size
A	cg18740861	1	1,78E–06	0.64	KNDC1	Body	0.97 (0.97–0.98)	−5.83e-04
A	cg21112943	2	1,07E–06	0.88	BAT4;CSNK2B	TSS20	0.03 (0.02–0.03)	5.82e-04
DHEA	cg16374953	1	2,06E–07	0.17	USPL1	TSS200	0 (0–0.01)	4.53e-01
DHEA	cg02327694	2	6,71E–08	0.06	LINC01340	Body	0.72 (0.69–0.75)	−1.68e-04
E1	cg11472319	1	5,62E–07	0.46	AGPAT3	TSS1500	0 (0–0.01)	2.08e-02
E1 (Total)	cg20992700	2	3,54E–07	0.29	MCTP1	Body	0.92 (0.9–0.93)	−1.62e-02
E2 (Total)	cg20513546	1	1,23E–07	0.06			0.91 (0.86–0.93)	−4.58e-02
E2 (Total)	cg07360763	2	4,37E–07	0.26	MIR646HG	Body	0.97 (0.96–0.97)	−3.36e-02
E2 (Free)	cg06070446	1	5,56E–08	<0.05	GALNT3	Body	0.94 (0.92–0.95)	1.44e+00
E2 (Free)	cg12034938	2	4,35E–07	0.36	CD84	Body	0.86 (0.84–0.87)	−6.42e-01
P4	cg15565234	1	1,42E–06	0.42	KIAA1462	5‘UTR	0.15 (0.13–0.18)	3.13e-03
P4	cg09535047	2	3,64E–07	0.30			0.96 (0.95–0.97)	−9.27e-03
SHBG	cg04615411	1	1,91E–06	1.00	GRM8	TSS200	0.02 (0.01–0.02)	3.79–01
SHBG	cg16656729	2	1,94E–07	0.16	C3orf20	Body	0.96 (0.94–0.97)	−3.80e-01
T (Total)	Cg16065899	1	1,72E–06	0.65	TLE1	5‘UTR;1stExon	0.11 (0.06–0.18)	−4.59e-01
T (Total)	cg20878647	2	1,93E–06	0.87	UBASH3A	Body	0 (0–0)	2.84e-04
T (Free)	cg08608892	1	2,43E–07	0.20			0.39 (0.32–0.47)	1.12e+00
T (Free)	cg01280180	2	2,31E–06	0.59	TXNDC6	5‘UTR	0.76 (0.73–0.8)	−4.84e-01

Androstenedione (A), dehydroepiandrosterone (DHEA), oestrone (E1), oestradiol (E2), progesterone (P4), sex hormone binding globulin (SHBG), testosterone (T).