Table 1.

Summary of Engineering Strategies of Phage Genomes

Phages	Year	Host Organism	Strategy	Editing	Efficiency	Reference
Andhra	2017	S. epidermidis	CRISPR-Cas10	Silent mutation	100%	[34]
AP205	2020	Acinetobacter	Synthesis and Gibson assembly	/	89.3%	[49]
BPs	2008	M. smegmatis	BRED strategy	200 nt deletion	15.8%	[28]
PaP1	2013	P. aeruginosa	In vivo recombination	Gene exchange	/	[22]
P2	2017	L. lactis	CRISPR-Cas9	Gene deletion	100%	[31]
phiKpS2	2018	K. pneumoniae	CRISPR-Cas9	1 kb deletion	100%	[32]
T4	2021	E. coli	CRISPR-Cas12a	11 kb deletion	100%	[35]
T7	2009	E. coli	Yeast-based platform	Gene exchange	>25%	[46]
T7	2014	E. coli	TypeI-E-CRISPR-Cas	Gene deletion	40%	[36]
T7	2019	E. coli	Gibson assembly	Gene insertion	100%	[43]
T7	2020	E. coli	λ Red-mediated recombination	Gene replacement	1.0%	[27]
ΦX174	2003	E. coli	Polymerase cycling assembly	/	/	[48]
ΦX174	2012	E. coli	Yeast-based platform	Full genome synthesis	44%	[57]
ICP1_2011_A	2016	V. cholerae	Type I-E CRISPR-Cas	2670 nt gene deletion	58%	[37]
2972	2014	S. thermophilus	CRISPR-Cas9	Point mutation	100%	[33]