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. 2023 Mar 8;9(1):vead018. doi: 10.1093/ve/vead018

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The independent discovery of a second Bangui virus strain. (A) Top (green): protein alignments performed between each genome segment of CHRB594 and Nyangole virus were scanned in twenty-five amino acid windows of the open reading frames. Dashed lines indicate the median per cent identity. Bottom (blue): NGS genome coverage plots for CHRB594. (B) RT-qPCR linearity for the L (left) and S (right) segments. The CHRB594 index case (bold curve) was detected in both channels at approximately C_t = 28, corresponding to a viral load of 3.2 log copies per liter. (C) Top (green): protein alignments between each genome segment of CHRB594 and Bangui virus (accession numbers: MK896632.1, MK896631.1, and MK896630.1). Gaps in alignment from the missing sequence in CHRB594 were filled with light green. Bottom: nucleotide alignments of full-length sequences highlighting transitions (gold) and transversions (purple), silent mutations (green), and non-silent mutations (red) in CHRB594 relative to Bangui virus.