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WT postsynaptic Ca2+ response to low-frequency stimulation. Evoked postsynaptic Ca2+ response to a 1 Hz stimulation. Genotype is WT; Is-GAL4, Mhc-SynapGCaMP6f/UAS-tdTom::Sec61β. In this and in all subsequent videos, Ca2+ sensor GCaMP is in green, ER marker tdTom::Sec61β in magenta; time is shown in seconds, and stimulation period (when present) as “STIM”; scale bar, 10 µm.
