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Autophagic activity in penumbra tissue in the (MCAO/R)24 h group in circadian rhythm after Per1 knock-out. A, B, Western blot analysis and quantification of LC3BII/LC3BI (***p < 0.001, n = 7 mice per group) and p62 (**p < 0.01, ***p < 0.001, n = 7 mice per group). C, Immunofluorescence analysis showed that LC3B levels did not significantly differ between groups in Per1−/− mice (scale bar = 200 µm). Data are shown as the mean ± SEM.
