Mouse brain penumbra GluA1 expression and autophagic activity in circadian rhythm in the sham and (MCAO/R)12 h groups. A, Western blot analysis and quantification of GluA1 at ZT5–ZT7 and ZT17–ZT19 in the sham and (MCAO/R)12 h groups (n = 6 mice per group). B, C, Western blot analysis and quantification of LC3BII/LC3BI (**p < 0.01, n = 6 mice per group) and p62 (**p < 0.01, *p < 0.05, n = 6 mice per group) levels in penumbra tissue in the ZT17–ZT19 and ZT5–ZT7 groups. Data are shown as the mean ± SEM.