Skip to main content
. 2023 Apr 4;21(4):e3002051. doi: 10.1371/journal.pbio.3002051

Fig 5. Regulation of aerobic gene transcription by Gre factors.

Fig 5

(A) Effect of recombinant Gre proteins in the in vitro transcription of the cydA gene in a reconstituted biochemical system. Transcription was measured by qRT-PCR using conditions identical to the ones described in Fig 4. Data are the mean ± SD (N = 6). *** p < 0.001 as determined by one-way ANOVA. (B) Pausing of in vitro transcription reactions containing cydA templates was visualized in 7M urea-16% PAGE gels of RNA products labeled with α32P-UTP as described in Fig 4. Consumption of O2 (C) by log phase Salmonella grown in MOPS-GLC medium (pH 7.2) was measured polarographycally in an APOLLO 4000 free radical anlyzer equipped with an ISO-OXY/HPO O2 probe. Data are the mean ± SD (N = 6). **** p < 0.0001 as determined by unpaired t test. Reduced and oxidized nicotinamide adenine nucleotide (D) and intracellular ATP (E) were recorded in Salmonella grown aerobically to an OD600 of 0.25 in MOPS-GLC medium (pH 7.2) at 37°C. Data are the mean ± SD (D, N = 5; E, N = 10). **** p < 0.0001 as determined by unpaired t test. qRT-PCR, quantitative real-time PCR; WT, wild-type.