Table 3. Reference gene stability value ranked by NormFinder algorithm in liver and spleen of laying hens of the 80 weeks of production.
| Tissue | CC | CF | CC-CF | The best combination of two genes | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Genes | Stability value | ||||
| Liver | 1 | ACTB | 0.31 | 1 | ACTB | 0.22 | 1 | ACTB | 0.08 | ACTB and HMBS | 0.086 |
| 2 | GAPDH | 0.36 | 2 | HMBS | 0.29 | 2 | HMBS | 0.12 | |||
| 3 | HMBS | 0.39 | 3 | GAPDH | 0.30 | 3 | GAPDH | 0.16 | |||
| 4 | RNA18S | 0.75 | 4 | RNA18S | 0.35 | 4 | RNA18S | 0.25 | |||
| Spleen | 1 | GAPDH | 0.09 | 1 | HMBS | 0.17 | 1 | ACTB | 0.08 | ACTB and GAPDH | 0.111 |
| 2 | ACTB | 0.15 | 2 | ACTB | 0.18 | 2 | GAPDH | 0.13 | |||
| 3 | HMBS | 0.20 | 3 | GAPDH | 0.20 | 3 | HMBS | 0.21 | |||
| 4 | RNA18S | 0.34 | 4 | RNA18S | 0.49 | 4 | RNA18S | 0.39 | |||
CC: conventional cage; CF: cage-free; CC-CF: overall data.