Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Mus musculus) | 3T3-L1; murine adipocytes | American Type Culture Collection | CL-173; RRID: CVCL_0123 | |
| Biological sample (Sus scrofa domesticus) | DFAT cells; porcine adipocytes | This paper | Subcutaneous fat (pork belly) | Isolation technique outlined in Materials and methods section |
| Biological sample (Bos taurus) | Beef fat | This paper | Pectoralis minor (brisket point cut) | Obtained from local butcher shop |
| Biological sample (Gallus gallus domesticus) | Chicken fat | This paper | Chicken thigh | Obtained from local butcher shop |
| Biological sample (S. scrofa domesticus) | Pork fat | This paper | Subcutaneous fat (pork belly) | Obtained from local butcher shop |
| Biological sample (S. scrofa domesticus) | Lard (Porcine) | Goya | – | |
| Biological sample (B. taurus) | Tallow (Bovine) | Epic Provisions | – | |
| Other | 300 µm cell strainer | pluriSelect | 43-50300-03 | For primary cell isolation (separating cells from debris) |
| Other | Collagenase | Worthington Biochemical | LS004176 | For primary cell isolation (digesting ECM) |
| Chemical compound, drug | DMEM | Thermo Fisher | 10569044 | |
| Chemical compound, drug | Advanced DMEM/F12 | Thermo Fisher | 12634-028 | |
| Chemical compound, drug | GlutaMAX | Thermo Fisher | 35050-061 | |
| Other | Bovine calf serum | Sigma-Aldrich | 12,133C | Culture media component |
| Other | Fetal bovine serum (FBS) | Thermo Fisher | A31606-01; Lot: 2129571 |
Culture media component |
| Chemical compound, drug | Antibiotic/antimycotic | Thermo Fisher | 15240062 | |
| Chemical compound, drug | Primocin | InvivoGen | ant-pm-1 | |
| Other | Accumax | Innovative Cell Technologies | AM105 | Cell detachment reagent |
| Other | NucleoCounter | Chemometec | NC-200 | Cell counter |
| Peptide, recombinant protein | Insulin | Sigma-Aldrich | I0516 | |
| Chemical compound, drug | Dexamethasone | Sigma-Aldrich | D4902 | |
| Chemical compound, drug | IBMX | Sigma-Aldrich | I5879 | |
| Chemical compound, drug | Rosiglitazone | TCI America | R0106 | |
| Chemical compound, drug | Intralipid | Sigma-Aldrich | I141 | |
| Chemical compound, drug | Biotin | TCI America | B04631G | |
| Chemical compound, drug | Calcium-D-pantothenate | TCI America | P001225G | |
| Other | Stereolithography 3D Printer | Formlabs | Form 2 | For making custom molds when producing 3D cultured fats |
| Chemical compound, drug | Biocompatible 3D printing resin | Formlabs | Surgical guide resin | |
| Chemical compound, drug | Medical device adhesive | Henkel Adhesives | Loctite 3556 | |
| Chemical compound, drug | Sodium alginate | Modernist Pantry | 1007-50 | |
| Chemical compound, drug | Calcium carbonate | Modernist Pantry | 1505-50 | |
| Chemical compound, drug | Glucono delta-lactone | Modernist Pantry | 1159-50 | |
| Peptide, recombinant protein | Microbial transglutaminase (mTG) | Ajinomoto | Activa TI | |
| Other | Goat serum | Thermo Fisher | 16210-064 | For use during immunofluorescent staining |
| Chemical compound, drug | BODIPY 493/503 | Invitrogen | D3922 | |
| Chemical compound, drug | DAPI | Thermo Fisher | 62248 | |
| Chemical compound, drug | Hoescht 33342 | Invitrogen | H3570 | |
| Antibody | Anti-fatty acid synthetase (rabbit, monoclonal) | Invitrogen | MA5-14887; RRID: AB_10980075 | (1:50) |
| Antibody | Anti-peroxisome proliferator-activated receptor gamma (rabbit, polyclonal) | Abcam | ab45036; RRID: AB_1603934 | (1:500) |
| Antibody | Anti-rabbit Alexa Fluor plus 647 (goat, polyclonal) | Invitrogen | A32733; RRID: AB_2633282 | (1:500) |
| Chemical compound, drug | Mounting media | Vector Laboratories | H-1700 | |
| Software, algorithm | Image analysis/quantification | CellProfiler (https://cellprofiler.org) | CellProfiler 4.2.1; RRID: SCR_007358 | |
| Other | Cell scraper (large) | Sarstedt | 83.3952 | For harvest and aggregation of differentiated adipocytes |
| Chemical compound, drug | Methyl tert-butyl ether | Alfa Aesar | 41839AK | |
| Chemical compound, drug | Methanol | Thermo Fisher | BPA4544 | |
| Software, algorithm | Graphing and statistical software | GraphPad Prism (https://www.graphpad.com) | GraphPad Prism 9.3.0; RRID: SCR_002798 |