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. 2023 Mar 1;55(3):520–531. doi: 10.1038/s12276-023-00947-9

Fig. 3. TGFBI KO mice show increased adipsin expression and macrophage infiltration in adipose tissue.

Fig. 3

A Gene set enrichment (left) and expression heatmap (right) in iWAT obtained from 20-week-old HFD-fed WT and KO mice. B-C Adipsin expression levels were verified by performing western blot analysis (upper), qRT‒PCR (bottom, left), and ELISAs (bottom, right) in iWAT obtained from the ND-fed (B) and HFD-fed (C) WT and KO mice. D Secretory (left) and mRNA expression of adipsin (right) in differentiated BM-MSCs isolated from the WT and KO mice. E Cy7-CD45-positive cells were isolated from iWAT of the WT and TGFBI KO mice and identified by FACS. F-G FITC-CD11b- (F) and APC-CD206-positive (G) cells were verified and quantified (right). H Relative mRNA levels of the indicated genes in iWAT obtained from the above mice. Error bars represent the ± SEM. *p < 0.05 by two-sided t test.