Fig. 6.

tG026 inhibits the interaction between TSR1 and 18S rRNA to reduce GPS. a The schematic of using RNA pull-down binding LC-MS to screen interactive proteins with tG026. b Venn diagram showing the interacting proteins with tG026 common to both HL-7702 and AML12 after tG026 RNA pull-down. The screening standards of interacting proteins are as follows: 1. The average value in control group must be zero; 2. The average value in tG026 group is non-zero. The red font represents the number of interacting proteins common to HL-7702 and AML12. n = 3 replicates. c, d RNA pull-down verified the interaction between TSR1 and tG026 in HL-7702 (c) and AML12 (d). e, f After tG026 and Flag-TSR1 were transfected, the interaction between TSR1 and 18S rRNA in HL-7702 (e) and AML12 (f) were detected by RIP. Input showing the unchanged 18S rRNA expression after transfection of tG026. n = 3 replicates. g, h Global protein synthesis under H₂O₂ stress in HL-7702 (g) and AML12 (h) was assayed by OP-Puro incorporation. Representative images (left panel) and statistical results (right panel) are shown. Scale bar, 50 μm. n = 3 replicates. All data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, n.s. no significance, Student’s t-test