Table 2.
Retention time, molecular weight and mass spectral characteristics of BAs, sterols and stanols (analyzed as TMS derivatives).
| Compound | Retention time (min) | Molecular weight (g/mol) | Characteristic Fragment ions (m/z) | ||
|---|---|---|---|---|---|
| Target ion | Qualifier ion 1 | Qualifier ion 2 | |||
| LCA-d4 | 15.3 | 380.6 | 434.4 | 261.3 | 219.2 |
| LCA | 15.3 | 376.6 | 430.4 | 257.3 | 215.2 |
| HDCA | 15.8 | 392.6 | 345.3 | 428.4 | 255.2 |
| UDCA | 15.8 | 392.6 | 518.4 | 428.4 | |
| DCA | 16.4 | 392.6 | 428.4 | 345.3 | 256.2 |
| CDCA | 16.8 | 392.6 | 413.3 | 428.4 | 255.2 |
| Methandienone | 12.8 | 300.4 | 122.1 | 161.2 | 242.2 |
| Epicoprostanol/coprostanol | 14.3 | 388.7 | 370.4 | 355.4 | 215.2 |
| Cholesterol | 14.7 | 386.7 | 458.4 | 368.4 | 329.3 |
| DHC | 14.8 | 388.7 | 445.4 | 460.5 | 215.2 |
| Stigmasterol | 15.5 | 412.7 | 484.5 | 394.4 | 255.2 |
| β-Sitosterol | 15.9 | 414.7 | 486.5 | 396.4 | 357.4 |
| β-Sitostanol | 16.0 | 416.7 | 473.5 | 488.5 | 383.4 |