Table 4.
Intra-day precision, accuracy (bias%), and recovery for each compound.
| Analyte | Precision | Accuracy (bias%) | Recovery (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 5 pg/mg | 25 pg/mg | 125 pg/mg | 5 pg/mg | 25 pg/mg | 125 pg/mg | 5 pg/mg | 25 pg/mg | 125 pg/mg | |
| LCA | 10.2 | 6.8 | 0.8 | 12.2 | 6.5 | 0.9 | 41.8 | 32.4 | 40.9 |
| HDCA | 7.2 | 5.3 | 2.1 | 9.6 | 4.7 | 2.2 | 39.3 | 49.3 | 48.7 |
| UDCA | 11.7 | 9.5 | 2.1 | 15.5 | 8.7 | 2.2 | 30.8 | 35.5 | 37.8 |
| DCA | 10.1 | 12.8 | 4.7 | 11.1 | 12.4 | 4.6 | 27.0 | 35.0 | 36.5 |
| CDCA | 10.9 | 11.6 | 2.6 | 11.0 | 11.7 | 2.7 | 45.5 | 38.0 | 48.6 |
| Epicoprostanol/coprostanol | 12.7 | 4.8 | 1.4 | 14.2 | 4.8 | 1.4 | 44.1 | 48.3 | 38.8 |
| Cholesterol | 11.4 | 8.7 | 1.5 | 13.0 | 8.5 | 1.6 | 62.0 | 73.1 | 61.9 |
| DHC | 12.5 | 11.7 | 2.3 | 13.2 | 11.3 | 2.3 | 33.2 | 32.5 | 38.7 |
| Stigmasterol | 13.0 | 11.5 | 3.1 | 15.0 | 11.2 | 3.2 | 36.0 | 43.9 | 40.0 |
| β-Sitosterol | 9.9 | 9.6 | 4.7 | 11.9 | 8.8 | 4.8 | 53.1 | 85.3 | 61.8 |
| β-Sitostanol | 12.0 | 7.8 | 1.5 | 14.7 | 7.6 | 1.5 | 45.6 | 32.5 | 45.6 |