Fig. 5. Lipidomics and metabolomics analysis of pediatric cohorts reveal alterations in nutrient absorption and metabolism and increases in inflammatory mediators.

A Workflow showing targeted and untargeted assays used to interrogate lipid and metabolite changes associated with acute pediatric COVID + and MIS-C. B Unbiased hierarchal clustering using all confidently annotated analytes. C Overlap between each pairwise comparison with respective statistical criterion. D Principal component analysis (PCA) using all confidently annotated analytes. E Top 10 analytes that define separation of principal component one. F Log 2 fold change of significant features present in both COVID + and MIS-C (p-adjusted value <0.1) show changes in inflammatory mediators (Limma, Benjamini-Hochberg correction). G Bubble Plot of KEGG pathway analysis comparing groups highlights unique aspects of MIS-C using analytes with a p-value <0.05 and DAPs determined from proteomic analysis (Fisher Exact test). Source data are provided as a Source Data file.