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. 2023 Mar 3;55(3):574–586. doi: 10.1038/s12276-023-00955-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A BODIPY staining of LDs in the choroid plexus (marker with AE2). Scale bar = 20 μm. B Representative images of PLIN3 staining indicating LDs in the choroid plexus. Scale bar = 50 μm. C TEM images showed tight junctions and LDs in the choroid plexus. Scale bar = 1 μm. D LD area percentage of total cytoplasm according to TEM images. **P < 0.01, sham (WT) versus ICH-IVH (WT); ##P < 0.01, ICH-IVH (WT) versus others (n = 3, one-way ANOVA). E Immunofluorescence staining of ZO-1 and claudin-5 in the choroid plexus after inhibiting LDs. Scale bar = 20 μm. F CSF albumin content after ICH-IVH with or without CAY10650 treatment (n = 6, unpaired t test). The results are expressed as the mean ± SD, **P < 0.01.