Figure 3.

Loss of PRC2 impairs the RNA scanning process and PRC2 interacts with Ptiwi09

(A–D) sRNAs of EV and Ezl1 KD in both early and late time points.

(E) Ptiwi09-GFP localization in the late stage on EV and PtCAF1 KD.

(F) PtCAF1-GFP localization in the late stage on EV and Ptiwi01/09 KD.

(G) Western blot of HA-immunoprecipitated proteins using anti-HA or anti-Myc antibodies to detect PtCAF1-FLAG-HA and Ptiwi09-Myc.

(H) Western blot of HA-immunoprecipitated proteins using anti-HA or anti-Myc antibodies to detect Ptiwi09-FLAG-HA and PtCAF1-Myc on Pgm KD and without KD. Samples are from the late time point.

Scale bars: 10 μm. EV, empty vector silencing; IES, internally eliminated sequence; MDS, macronuclear destined sequence (MAC matching); no KD, no silencing; OES, other eliminated sequence; TE, transposable element. See also Figure S6.