Figure 7.

LFA-evoked grasping does not require RFA activation

(A) Representative MIPs showing optogenetically evoked cortical activation in LFA during RFA topical application of vehicle (left) and CNQX (right). Cross represents the stimulus site. Red dashed line indicates the CNQX topical application site. Black dot represents bregma. Scale bar, 1 mm.

(B) Quantification of the effect of CNQX topical application in RFA on LFA MSAM extension (vehicle 0.12 ± 0.01 mm² vs. CNQX 0.11 ± 0.02 mm²; n = 3; paired-sample t test).

(C) Averaged LFA evoked calcium transient profiles in vehicle and following CNQX topical application in RFA (vehicle 15 ± 2 ΔF/F vs. CNQX 13 ± 1 ΔF/F; n = 3; paired-sample t test). Shading represents SEM.

(D) Representative activity propagation maps of GRASP LFA showing the effect of CNQX topical application in RFA.

(E) Pixel rank distribution of the region corresponding to the LBMM (bright area in [D]) for GRASP LFA, before (vehicle) and after CNQX topical application (n = 3). med, median; IQR, interquartile range; Wilcoxon signed-rank test.

(F) Effects of RFA CNQX topical application on GRASP LFA kinematics. Comparison of the absolute left forelimb maximum elevation (left) and the maximum lateral displacement (right) following LFA stimulation in vehicle and RFA CNQX topical application (maximum elevation, vehicle 4.9 ± 1.8 mm vs. CNQX 3.7 ± 1.4 mm; lateral displacement, vehicle 7.7 ± 2.0 mm vs. CNQX 1.3 ± 1.0 mm; n = 3; paired-sample t test). Red lines indicate means, boxes show the standard error range, and whisker length represents the extreme data points.