Table 1.
List of reagents, resources, and tools used for the manuscript.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Biotinylated CD3 | STEMCELL Technologies | 18051 |
| Anti-RBC | Rockland | 1094139 |
| CD107a | Biolegend | 328625 |
| Viability | Tonbo | 13-0865-T500 |
| FcRγ | Millipore | FCABS400F |
| PLZF | Invitrogen | 17-9322-82 |
| CD247 (CD3ζ) | Biolegend | 644105 |
| IFNγ | BD Biosciences | 562392 |
| TNFα | Biolegend | 502938 |
| SYK | BD Horizon | 624144 |
| ZAP-70 | Biolegend | 691206 |
| CD16A | Biolegend | 360728 |
| SHP-1 | abcam | Ab209913 |
| CD56 | Beckman Coulter | A79388 |
| CD3 | Biolegend | 317322 |
| CD45 #1 BV510 | Biolegend | 304036 |
| CD45 #2 BV650 | Biolegend | 304043 |
| CD45 #3 BV785 | Biolegend | 304048 |
| CD45 AF647 | Biolegend | 304056 |
| CD11a (TS2/4) | Biolegend | 350606 |
| CD11a/CD18 (m24) | Biolegend | 363408 |
| CD18 (TS1/18) | Biolegend | 302108 |
| CD2 | Biolegend | 300224 |
| Trastuzumab (anti-HER2) | N/A | |
| Rituximab (anti-CD20) | N/A | |
| Biological samples | ||
| Healthy adult whole blood | Memorial Blood Bank | N/A |
| RPMI-1640 | Kd Medical | 50-101-8907 |
| Ficoll | MP Biomedicals | ICN50494 |
| ACK Lysis Buffer | Lonza | BP10-548E |
| Fetal bovine serum | PEAK Serum | PS-FB1 |
| EDTA | Fisher | BP2482-1 |
| RPMI-1640 | FIsher | SH3002701 |
| Gentamicin | Sigma-Aldrich | G1272 |
| Trypsin EDTA | Gibco | 25300062 |
| McCoy’s 5A Medium | Gibco | 166600082 |
| Pen/Strep | Gibco | 1514022 |
| MEM-alpha | Gibco | 12561 |
| HI-FBS | Gibco | 16140071 |
| HI-Horse Serum | Gibco | 26050088 |
| IL-2 | NCI | N/A |
| 2-Mercaptoethanol | Gibco | 21985023 |
| X-VIVO 15 | Lonza | BE02-060F |
| IL-15 | NCI | N/A |
| Cas9 | IDT | 1081059 |
| P3 Primary Cell Solution | Lonza | V4XP-3032 |
| BFA | Millipore Sigma | 20350-15-6 |
| Monensin | Millipore Sigma | M5273 |
| Formaldehyde | Thermo | 28908 |
| Triton | Fisher | BP151-500 |
| BSA | MP Biomedicals | 810681 |
| Critical commercial assays | ||
| NK Cell Isolation Kit | STEMCELL Technologies | 17955 |
| DNEasy Kit | Qiagen | 69506 |
| LEGENDScreen Human PE Kit | Biolegend | 700007 |
| Cell Trace Violet Labeling Kit | Thermo Fischer | C34571 |
| Cell Trace Far Red Labeling Kit | Thermo Fischer | C34564 |
| DELFIA | PerkinElmer | AD0116 |
| Experimental models: Cell lines | ||
| K562-aAPC | Branden Moriarity laboratory | K562-aAPC |
| SKOV-3 | Jeff Miller laboratory | SKOV-3 |
| SKOV-3 NLR | Jeff Miller laboratory | SKOV-3 NLR |
| Raji NLR | Jeff Miller laboratory | Raji NLR |
| NK-92-GFP | Bruce Walcheck laboratory | NK-92-GFP |
| NK-92-GFP-CD16A | Bruce Walcheck laboratory | NK-92-GFP-CD16A |
| NK-92-GFP-CD64/16A | Bruce Walcheck laboratory | NK-92-GFP-CD64/16A |
| Oligonucleotides | ||
| See supplementary table 1 | ||
| Software and algorithms | ||
| CRISPR Design Tool | Synthego | https://design.synthego.com/#/ |
| FlowJo (10.8.0) | FlowJo | N/A |
| ICE Analysis | Synthego | https://ice.synthego.com/#/ |
| Incucyte base analysis software | Sartorius | https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-software/incucyte-base-software#id-786768 |
| Excel (16.54) | Microsoft | N/A |
| RStudo (1.4.1717) | N/A | N/A |
| Prism (9.1.2) | GraphPad | N/A |
| Illustrator (25.4.1) | Adobe | N/A |
| Other | ||
| Red blood cell purification filter | Memorial Blood Center | 4C4300 |
| Mr. Frosty | Thermo-FIsher | 5100-0001 |
| G-Rex 6-well plates | Wilson Wolf | 80240M |
| G-Rex 24-well plates | Wilson Wolf | 80192M |