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. Author manuscript; available in PMC: 2023 Apr 5.
Published in final edited form as: Nat Chem. 2023 Jan 12;15(4):526–534. doi: 10.1038/s41557-022-01117-6

Fig. 3 |. CtdP active site view and mutagenesis study.

Fig. 3 |

a, Active site of CtdP. The CtdP residues are shown in blue, while NADP+ and 10 are shown in yellow and salmon, respectively. b, Superposition of the common bicyclo[2.2.2]diazaoctane ring in (+)-11 (green) with that in 10 (salmon) in CtdP-NADP+−10 complex. c, Analysis of the formation of 10 with CtdP mutants relative to wild-type (WT) production. Data represents as mean ± s.d. from triplicate independent experiments (n = 3). The relative ratio of product 2 formation in the mutant compared to the WT CtdP is based on the peak area of UV at 280 nm.