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. 2022 Aug 16;40(7):111230. doi: 10.1016/j.celrep.2022.111230

Figure 4.

Figure 4

Gene expression within fibroblast foci

(A) Heatmap showing top differentially expressed genes between IPF fibroblastic foci and all other ROIs.

(B) TNC expression.

(C) CRABP2 expression.

(D) COL1A2 expression.

(E) COMP expression.

(F) Collagen fibril organization GSVA scores.

(G) COL4A3 expression.

(H) Bone morphogenesis GSVA scores.

(I) PLOD2 expression.

(J) RUNX1 expression. Statistical comparisons are relative to control alveolar septae. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 by Wilcoxon test with Benjamini-Hochberg multiple test correction.

(K) Representative multiplexed RNA in situ hybridization for COMP, COL1A2, and CRABP2 expression with a fibroblastic focus identified by ∗, with the corresponding fibroblast focus (∗) identified by H&E staining on the same tissue section. Scale bar, 100 μm. Inset scale bar, 50 μm.