Figure 3.

Early elevation of pNF-H expression observed in the retina and ONH following induction of AOH. An obvious elevation of the neurofilament was also observed in the retina. (A) Representative blots of NF-H, M, L, and pNF-H from AOH and the control retinas at 4, 6, and 12 h are shown. In the retina, the bands of the neurofilament three subunits were more visible at 6 and 12 h, while the band of pNF-H was more visible as early as 4 h. pNF-H, NF-H was recognized at 200 kDa, NF-M was visible at 160 kDa, and NF-L was at 70 kDa. GAPDH was used as an internal control. (B) The expression of neurofilament subunits and pNF-H in the ONH was traced at 4, 6, and 12 h after AOH, but western blotting of the ONH showed that NF-H, NF-M, and NF-L were unchanged, while the pNF-H band increased significantly at 6 h. (C) Densitometry measurements of three neurofilament subunits in the retina (normalized for GAPDH and expressed relative to the control) are provided in the box-and-whisker plots, n = 5 mice for each group, **P < 0.01, ***P < 0.001. (D, E) Quantification of pNF-H expression from the retina at 4 h and ONH at 6 h is shown as box-and-whisker plots, respectively. n = 5 mice for each group, *P < 0.05, ***P < 0.001.