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. 2023 Mar 22;14:1091697. doi: 10.3389/fneur.2023.1091697

Figure 4.

Figure 4

NF-H and its phosphoforms accumulated in the soma of peripheral RGCs after AOH. (A) Immunofluorescence of the pNF-H marker, SMI31, in the retinal wholemount 4 h after AOH; retinal wholemount was observed from peripheral to central regions. Asterisk indicates pNF-H accumulation in the peripheral retina. Scale bars: 50 μm. (B) Quantification of pNF-H accumulation in the peripheral retina. The data are presented as a scatter diagram. Mean ± SD is shown, n = 5 for each group, ***P < 0.001. (C) Immunofluorescence of pNF-H and NF-H in the retinal wholemount of AOH after 6 h. Micrographs show that most of the pNF-H accumulations were overlapped with NF-H, with arrows indicating examples of co-expression, and the high magnification image is also shown (top right of the third picture), Scale bars: 50 μm. (D) Immunofluorescence of pNF-H and the RGC markers Brn3a and NeuN in the retinal wholemount of AOH after 6 h. pNF-H accumulations were highly overlapped with an RGC marker, and the high magnification images are also shown (top right of the third picture and sixth picture). Scale bars: 20 μm.