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Real-time PCR primers.
| Gene | Forward primer sequences (5’-3’) | Reverse primer sequences (5’-3’) |
|---|---|---|
| β-actin | CTACACCGCTACCAGTTCGC | TAGGAGTCCTTCTGGCCCAT |
| NLRP3 | TTGTGTGGACCTATGCCCTG | GAGAGATGCAGCCCTTCTGA |
| Caspase-1 | ATCAATTCGCACACGCCTTG | TATAGCCATGTCCGAAGCGG |
| IL-18 | GCTGCTGAACCGGAAGACAA | AAACACGGCTTGATGTCCCT |
| ASC | GCTGGAATCAAAGCCCTTCC | TCCACGTCTGTGACCCTTGA |
| IL-1β | AAGATAACACGCCCACCCTG | GAGTTTCCCAGGAAGACGGG |
