TABLE 3.
The medium used for SC-CM maturation.
| Major supplement | CM purity | Circularity index | PPAR expression | MCF (mN/mm2) | APD (ms) | References |
|---|---|---|---|---|---|---|
| 10 mM galactose, 100 µM OA, 50 μM PA | >80% | ∼0.3 | increased | 0.4 | APD50: 580 | Correia et al. (2017) |
| fatty acid + T3 | >90% | — | increased by 5-fold | — | APD50: 450 | Lin et al. (2017) |
| LA-OA-albumin | 98% | ∼0.5 | increased by 2-fold | — | — | Horikoshi et al. (2019) |
| 105 μM PA-albumin, 81 μM OA-albumin, 45 μM LA-albumin | >80% | ∼0.5 | — | 14 (nN/cell) | APD50: 282 | Yang et al. (2019) |
| L-lactate, lipid-rich BSA | 95% | — | — | — | increased | Feyen et al. (2020) |
| galactose, OA, LA | >75% | ∼0.2 | increased | 33 | — | Knight et al. (2021) |
| 10 mM galactose, 125 µM OA, 100 μM PA | 80% | — | — | ∼5 | APD80: 420 | Huebsch et al. (2022) |
APDx, refers to action potential duration at x% repolarization. BSA, bovine serum albumin; CM, cardiomyocyte; LA, linoleic acid; MCF, maximum contractile force; OA, oleic acid; PA, palmitic acid; PPAR, peroxisome proliferator-activated receptor; T3, tri-iodothyronine.