Table 2.
Primers used for Bovine ephemeral fever virus (BEFV) genome amplification in this study.
| Primer pairs | Sequence (5′–3′) | Locationsa | Length of amplicon (bp) |
|---|---|---|---|
| 1 | ACGAGAAAAAACAAAAAAACTAATTGATA | 1–29 | 2,728 |
| GCAGTTCCGGTGAATTCTATTACCTCG | 2,702–2,728 | ||
| 2 | TTCAAAACCAATAGAAAGAACAAC | 2,522–2,545 | 1,698 |
| TAAGATCCGATCCCATAATGAT | 4,198–4,219 | ||
| 3 | ACAGATAGAACAGAATTTGAAG | 3,921–3,942 | 1754 |
| GCTTAATCAACTCTAGTCTAAT | 5,653–5,674 | ||
| 4 | ATTATCCTCCTCCAAAGTGCGA | 5,393–5,414 | 1,560 |
| TCAATATAATCCAAAATCCTAG | 6,931–6,952 | ||
| 5 | AACAGGCAATGGAGAAAGG | 6,815–6,833 | 2,368 |
| GTTTGAAACCTGCTAATTA | 9,164–9,182 | ||
| 6 | GGACATAAGTGGACGGTGGATA | 9,063–9,084 | 1967 |
| TTTCTCCTCTGATTGCTGGATT | 11,008–11,029 | ||
| 7 | GGAACAATAAAGGGACTGCCAA | 10,807–10,828 | 2018 |
| GTGAACTCTGATATAAATGATG | 12,803–12,824 | ||
| 8 | GGTAGGTGATTCACTGTTAAGT | 12,582–12,603 | 1,375 |
| AGCATGTCTAATATATTTGTA | 13,935–13,956 | ||
| 9 | GAAACAGAATTCTATGATGATTGACCTGAT | 13,767–13,788 | 1,133 |
| ACGAAGAAAAACAAATAAAATACAATTCCT | 14,870–14,899 |
a
The location is determined based on the representative strain Bovine/China/Henan1/2012 (KM276084.1).