Table 3.
Major fatty acids of strain RST and its closely related strains in the genus Ralstonia. Strain: 1. RST; 2. GMI1000; 3. CQPS-1; 4. R. pseudosolanacearum LMG 9673T; 5. R. solanacearum LMG 2299T; 6. R. syzygii subsp. syzygii LMG 10661T; 7. R. syzygii subsp. celebesensis LMG 27706T; 8. R. syzygii subsp. indonesiensis LMG 27703T.
| Fatty acids | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
|---|---|---|---|---|---|---|---|---|
| Saturated: | ||||||||
| C14:0 | 4.28 ± 0.26 | 3.42 ± 0.03 | 3.39 ± 0.04 | 5.03 ± 0.22 | 5.05 ± 0.34 | 1.88 ± 0.42 | 6.55 ± 0.56 | 4.74 ± 0.12 |
| C16:0 | 30.99 ± 2.98 | 24.26 ± 1.33 | 25.30 ± 0.28 | 22.45 ± 1.59 | 29.85 ± 2.28 | 38.71 ± 1.85 | 34.63 ± 1.20 | 27.25 ± 0.93 |
| C18:0 | 2.03 ± 0.97 | 8.97 ± 1.29 | 9.32 ± 0.16 | 3.21 ± 1.93 | 3.16 ± 1.28 | 14.09 ± 1.21 | 5.92 ± 1.78 | 1.76 ± 1.64 |
| Cyclo-C17:0 | 4.57 ± 1.18 | 2.15 ± 0.19 | 2.10 ± 0.11 | 2.84 ± 1.51 | 7.11 ± 1.29 | 7.51 ± 0.36 | 15.70 ± 1.99 | 3.14 ± 0.57 |
| Unsaturated: | ||||||||
| 18:1 ω9c | 4.54 ± 0.84 | 2.43 ± 1.03 | 2.09 ± 0.52 | 2.82 ± 0.63 | 3.97 ± 0.09 | – | – | 2.69 ± 1.18 |
| Hydroxy: | ||||||||
| iso-C11:0 3-OH | 1.90 ± 0.42 | TR | TR | 1.04 ± 0.32 | TR | – | – | 1.00 ± 0.02 |
| C16:0 2-OH | 1.19 ± 0.16 | – | – | TR | 1.31 ± 0.31 | 0.46 ± 0.08 | 2.01 ± 0.18 | TR |
| C16:1 2-OH | 3.39 ± 0.59 | TR | TR | 3.71 ± 1.88 | TR | 0.84 ± 0.12 | 4.65 ± 0.67 | 1.46 ± 0.05 |
| C18:1 2-OH | 5.30 ± 0.36 | 4.23 ± 0.19 | 4.34 ± 0.01 | 4.39 ± 0.53 | 3.62 ± 0.92 | 3.15 ± 0.24 | 4.28 ± 0.65 | 3.05 ± 0.54 |
| Summed features a : | ||||||||
| 2 | 7.89 ± 0.79 | 6.99 ± 0.25 | 6.34 ± 0.21 | 8.40 ± 1.05 | 10.16 ± 0.59 | 5.96 ± 0.35 | 15.36 ± 1.79 | 9.61 ± 0.18 |
| 3 | 19.12 ± 0.94 | 22.84 ± 0.82 | 21.10 ± 0.48 | 24.76 ± 3.26 | 20.76 ± 0.22 | 2.74 ± 0.08 | 5.19 ± 0.17 | 28.77 ± 2.07 |
| 8 | 13.70 ± 1.59 | 19.30 ± 1.31 | 21.05 ± 0.48 | 18.53 ± 0.98 | 11.70 ± 2.23 | 4.38 ± 0.43 | 5.72 ± 0.56 | 13.62 ± 1.33 |
The data are expressed as percentages of total fatty acids. TR, trace amount (less than 1.0% of the total); −, not detected.
Summed features are fatty acids that cannot be resolved reliably from another fatty acid using the chromatographic conditions chosen. The MIDI system groups these fatty acids together as one feature with a single percentage of the total. Summed feature 2 comprises C14:0 3-OH/C16:1 iso I. Summed feature 3 comprises C16:1 ω7c and/or C16:1 ω6c. Summed feature 8 comprises C18:1 ω7c and/or C18:1 ω6c.