Skip to main content
. 2023 Mar 22;14:1052925. doi: 10.3389/fimmu.2023.1052925

Figure 3.

Figure 3

Inhibiting deposition of epigenetic mark H3K27ac through p300 suppresses iNOS signaling. (A) RT-qPCR analysis of Nos2 in primed MMC, indicating that the inhibitor GNE-049 decreased transcriptional expression of Nos2 by inhibiting p300/CBP. Two individual experiments (n=3) were performed. (B, C) Immunofluorescence microscopy analyses of Nos2 in GNE-049 co-treated, primed MMCs (n=4). Scale bar, 10 µm. Immunoblotting analyses of (D, E) Nos2 (n=3). (F) RT-qPCR analysis demonstrated that the relative expression levels of IL-1β in p300 knockdown (KD) following LPS treatment, compared to MMC with negative control siRNA. (G) RT-qPCR analysis of Nos2 in primed MMC (n=4~12) following the treatment paradigm to examine the effects of GNE-049 on the transcription levels of Nos2 upon a secondary Mn insult. Data show mean ± SEM of one-way ANOVA followed by Tukey’s post hoc test. Ctrl, control; **p<0.01; ***p<0.001.