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. 2023 Mar 22;14:1052925. doi: 10.3389/fimmu.2023.1052925

Figure 4.

Figure 4

GNE-049 increases the expression of the M2 polarization marks IRF4 and ARG1. Representative Western blots and densitometry assessing (A, B) IRF4 (n=4) and (C, D) ARG1 (n=8) in MMCs. (E) MitoTracker probe staining shows changes in mitochondrial morphology in MMCs cotreated with GNE-049 (n=3). Scale bar, 10 µm. (F, G) MitoSOX assays reveal that GNE-049 reduced LPS-induced superoxide generation by inhibiting H3K27ac deposition (n=4). Scale bar, 10 µm. (H) JC1 probe (scale bar, 50 µm) and (I) quantitative analysis of Δψm by the ratio of red and green fluorescence. Data show mean ± SEM of one-way ANOVA followed by Tukey’s post hoc test. Ctrl, control; *p ≤ 0.05.