Figure 4.

Effect of HS-vri induction on sleep architecture. The effect of induction on sleep architecture was measured for four different groups (w¹¹¹⁸ and HS-vri males, pre- and post-induction). The average minutes of sleep/hour is plotted as a function of elapsed time. For both genotypes, sleep was measured under 12-h light:12-h dark conditions, indicated with the white and black horizontal bars. Flies were entrained to a 12:12 schedule and then loaded into the DAMS monitors maintained in two different 20°C incubators with identical settings. Baseline sleep was assayed for 2 days before one of the incubators was shifted to 28°C and sleep measurements continued for flies at both temperatures. The timing of the heat-shift is shown with the dotted horizontal line. The blue and red curves represent the amounts of sleep for the non-induced and induced flies respectively.