Table 1.
Effect of HS-vri induction on sleep.
| Males | ||||||||
|---|---|---|---|---|---|---|---|---|
| w1118 | Difference | HS-vri | Difference | |||||
| -HS N = 187 | +HS N = 184 | % | Sig. | -HS N = 187 | +HS N = 184 | % | Sig. | |
| Total sleep (min) | 846 | 1,067 | 21% | **** | 1,094 | 1,262 | 13% | **** |
| Day sleep (min) | 371 | 531 | 30% | **** | 391 | 557 | 30% | **** |
| Night sleep (min) | 475 | 549 | 13% | **** | 703 | 687 | −2% | **** |
| Total bout # | 31 | 40 | 23% | **** | 27 | 26 | −4% | ns |
| Day bout # | 17 | 20 | 15% | **** | 23 | 23 | 0% | ns |
| Night bout # | 14 | 19 | 26% | **** | 4.1 | 3.4 | −21% | * |
| Ave. bout L (min) | 37 | 33 | −12% | ns | 47 | 67 | +30% | **** |
| Day bout L (min) | 31 | 39 | 21% | * | 20 | 36 | +44% | **** |
| Night bout L (min) | 48 | 43 | −12% | * | 385 | 401 | +4% | ns |
w1118 and HS-vri flies (males) were used to assess the effect of a mild, chronic temperature shift (20°C to 28°C) on sleep. Flies loaded into the DAM apparatus were incubated in two different incubators set identically (20°C under 12:12 light:dark control). Once the flies were entrained, sleep was measured using the standard parameters for 2 days. One of the incubators was then shifted to 28°C at ZT = 12, and all groups were monitored for sleep for an additional 2 days. Each horizontal row of the Table measures a different sleep parameter: total, daytime, and nighttime sleep; total, daytime, and nighttime average number of bouts; total, daytime and nighttime average bout lengths. All values are shown in minutes. Genotypes and N size for each group are shown at the top. The Difference shows changes (positive or negative) in the induced vs. the uninduced group. The significance of the changes between the two groups is tabulated. *p < 0.05, ****p < 0.0001, ns = not significant.