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. 2023 Mar 22;14:1122125. doi: 10.3389/fendo.2023.1122125

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Copyright © 2023 Matsuno, Yokoe, Nagatsuka, Morihara, Moriwaki and Asahi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increased O-GlcNAcylation in the hearts of Ogt-Tg mice four weeks after TAC (TAC4W). (A) Western blot analysis for OGT, O-GlcNAc, GFAT1, and GFAT2 in heart tissues from WT and Ogt-Tg mice with or without TAC4W. Representative data was designated. (B–E) Quantifications of OGT, O-GlcNAc, GFAT1, and GFAT2 levels in (A) from 3 independent experiments using ImageJ software. The data were evaluated by two-way analysis of variance (ANOVA) followed by Tukey’s test. Values are shown as mean ± SD (*P<0.05). n.s., not significant; TAC, transverse aortic constriction; GFAT, glutamine-fructose-6-phosphate transaminase.