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. 2023 Mar 22;14:1122125. doi: 10.3389/fendo.2023.1122125

Figure 3.

Figure 3

Decreased phosphorylation of GSK-3β in the hearts of Ogt-Tg mice after TAC4W. (A) Western blot analysis for ANP, GSK-3β phosphorylation, and NF-κB phosphorylation in heart tissues from WT and Ogt-Tg with or without TAC4W. Representative data was designated. (B) Quantifications of ANP levels in (A) from three independent experiments using ImageJ software, and evaluated by two-way analysis of variance (ANOVA) followed by Tukey’s test. Values are shown as mean ± SD (n=3). *P<0.05. (C) The ratios of p-GSK-3β/GSK-3β expression intensity were quantified using ImageJ software, and evaluated by two-way analysis of variance (ANOVA) followed by Tukey’s test. Values are shown as mean ± SD value (n=3). *P<0.05. (D) The ratios of p-NF-κB/NF-κB expression intensity were quantified using ImageJ software, and evaluated by two-way analysis of variance (ANOVA) followed by Tukey’s test. Values are shown as mean ± SD value (n=3). TAC, transverse aortic constriction; ANP, atrial natriuretic peptides.