| Component | Amount |
|---|---|
| Purified gDNA template | Up to 2.5 μg |
| P5 primer mix (10 μM) | 1.25 μl |
| Barcoded P7 primer (10 μM) | 1.25 μl |
| 2× NEBNext Ultra II Q5 Master Mix | 25 μl |
| Molecular biology grade water | To 50 μl |
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