Table 1. Control sgRNA sequences.

Control	sgRNA sequence	Notes
Positive-1	TG GAAATATACATCAT AAG A	EEF2
Positive-2	TGCCAACCTCCGACAAAGGT	EEF2
Positive-3	GTTGTGCGCGTGCTCGAAGG	EEF2
Negative-1	CTGACGTGTCTGAAATGAGT	Non-targeting
Negative-2	ATTTCCCTACGGAGATATCC	Non-targeting
Negative-3	ATCAAGTCAGGTTATGCGGG	Non-targeting
Negative-4	GGATACCTGGGCCGACTTTC	Non-targeting
Negative-5	CTCCGTTATGTG G CATGAGA	Non-targeting
Negative-6	CATTGTTATG GCCTCCTCCG	Functionally neutral
Negative-7	GAAAACCTAAGCTAAGTGGT	Functionally neutral
Negative-8	CACGAGACCATATCTTTCAG	Functionally neutral
Negative-9	GAAACTGGGTCAAACGAAGG	Functionally neutral
Negative-10	AT AAGCCAGAT ACAAGACCG	Functionally neutral