Table 2. CRISPOR subpool barcodes and round 1 PCR primers for library amplification.

CRISPOR barcodes and associated primers were previously reported in (Canver et al., 2018).

#	Forward barcode (5’)	Reverse barcode (3’)	Forward primer	Reverse primer
1	CGGGTTCCGT	GCTTAGAATAGAA	CGGGTTCCGTGGAAAGG	TTCTATTCTAAGCGCCTTATTTTAACTTGC
2	GTTTATCGGGC	ACTTACTGTACC	GTTTATCGGGCGGAAAGG	GGTACAGTAAGTGCCTTATTTTAACTTGC
3	ACCGATGTTGAC	CTCGTAATAGC	ACCGATGTTGACGGAAAGG	GCTATTACGAGGCCTTATTTTAACTTGC
4	GAGGTCTTTCATGC	CACAACATA	GAGGTCTTTCATGCGGAAAGG	TATGTTGTGGCCTTATTTTAACTTGC
5	TATCCCGTGAAGCT	TTCGGTTAA	TATCCCGTGAAGCTGGAAAGG	TTAACCGAAGCCTTATTTTAACTTGC
6	TAGTAGTTCAGACGC	ATGTACCC	TAGTAGTTCAGACGCGGAAAGG	GGGTACATGCCTTATTTTAACTTGC
7	GGATGCATGATCTAG	CATCAAGC	GGATGCATGATCTAGGGAAAGG	GCTTGATGGCCTTATTTTAACTTGC
8	ATGAGGACGAATCT	CACCTAAAG	ATGAGGACGAATCTGGAAAGG	CTTTAGGTGGCCTTATTTTAACTTGC
9	GGTAGGCACG	TAAACTTAGAACC	GGTAGGCACGGGAAAGG	GGTTCTAAGTTTAGCCTTATTTTAACTTGC
10	AGTCATGATTCAG	GTTGCAAGTCTAG	AGTCATGATTCAGGGAAAGG	CTAGACTTGCAACGCCTTATTTTAACTTGC