Table 4. Validation statistics for a typical sgRNA library.
| Metric | Value |
|---|---|
| Total filtered reads | 1,081,037 |
| Total filtered reads perfectly matching a library sgRNA | 975,982 |
| Total filtered reads without a perfect match | 105,055 |
| Percentage perfect matches | 90.3% |
| Number of undetected library sgRNAs | 0 |
| Percentage of undetected sgRNAs | 0% |
| Skew ratio | 2.74 |