Skip to main content
. Author manuscript; available in PMC: 2023 Apr 5.
Published in final edited form as: Curr Protoc. 2022 Dec;2(12):e614. doi: 10.1002/cpz1.614

Table 4. Validation statistics for a typical sgRNA library.

Metric Value
Total filtered reads 1,081,037
Total filtered reads perfectly matching a library sgRNA 975,982
Total filtered reads without a perfect match 105,055
Percentage perfect matches 90.3%
Number of undetected library sgRNAs 0
Percentage of undetected sgRNAs 0%
Skew ratio 2.74