Skip to main content
. 2023 Feb 3;10(10):2205573. doi: 10.1002/advs.202205573

Figure 5.

Figure 5

MS147, but not the parent EED binder, EED226, or EED/PRC2 degrader, PROTAC 2, effectively suppresses the proliferation in K562, MDA‐MB‐231, and NCI‐H1299 cells. a–c) The effect of MS147, EED226 and PROTAC 2 on inhibiting the growth in K562 (a), MDA‐MB‐231 (b), and NCI‐H1299 (c) cells. The tested cell lines were treated with serial dilution of EED226, PROTAC 2 or MS147 for 5 days. Cell viability was determined using the CCK‐8 assay. The data shown represent the means ± SD from three independent experiments. d–f) The effect of MS147, EED226, and PROTAC 2 on degrading BMI1, RING1B, EED, EZH2, and SUZ12 and reducing the levels of H2AK119ub and H3K27me3 in K562 (d), MDA‐MB‐231 (e), and NCI‐H1299 (f) cells. The above three cell lines were treated with EED226, PROTAC 2 or MS147 at the indicated concentrations for 24 h (d) or 48 h (e,f). The protein levels of EED, BMI1, RING1B, H2AK119ub, EZH2, SUZ12, and H3K27me3 were determined using WB with H3 as the loading control. The WB results shown are representative of two independent experiments.