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. 2023 Feb 1;24(4):e55362. doi: 10.15252/embr.202255362

Figure 3. Mi‐2 knockdown in larval neurons results in extensive gene expression changes.

Figure 3

  1. Volcano plot showing significantly upregulated (green) and downregulated (purple) genes with fold‐change. Selected mis‐expressed genes are annotated.
  2. RNA‐seq tracks showing vas mRNA expression in third instar larval CNS (Y‐axis is RPKM).
  3. Heatmap showing relative changes in normalised read‐counts between Mi‐2 RNAi and control replicates for all significantly differentially expressed genes (P‐adj < 0.05, log2 fold change > 1 or < −1). Significantly more genes are upregulated (1467) than downregulated (397).
  4. Heatmap showing relative expression levels of upregulated genes across selected Drosophila tissues (full version in Appendix Fig S2).
  5. Enriched gene ontology terms for genes that are upregulated in Mi‐2 RNAi knockdown.
  6. Proportion of differentially upregulated genes that directly intersect with Mi-2, MEP-1, MTA-1like and HDAC1 peaks, and differentially upregulated genes intersecting with exclusively Mi-2/MEP-1 or all NuRD subunits.