Reagent/resource	Reference or source	Identifier or catalog number
Experimental models
501mel (human melanoma)	Dr. R. Halaban (Yale University School of Medicine, New Haven, USA)
WM266.4 (human melanoma)	ATCC	Cat# CRL‐1676
451Lu (human melanoma)	Drs. M. Herlyn and J. Villanueva (The Wistar Institute, Philadelphia, USA)
NHEM (Normal Human Epithelial Melanocyte from for skin)	BIOalternatives (Gençay, France)
NMRI nude mice (M. musculus)	Charles river
SHrN® hairless NOD.SCID mice (M. musculus)
Melanoma tissue microarray	US Biomax	Cat# ME1004c
Recombinant DNA
pcDNA3.1(+)‐Zeocin_eGFP	This study
pcDNA3.1(+)‐Zeocin_eGFP‐TRPV2 (human)	This study
NON SILENCING shRNA_pGIPZ (shRNA control)	Thermo Scientific/GE Dharmacon	Cat# RHS4346
hTRPV2 shRNA_pGIPZ (shRNA V2‐1)	Thermo Scientific/GE Dharmacon	Cat# RHS4531‐EG51393 cloneID#RHS4430‐200207433‐V2LHS_97630
hTRPV2 shRNA_pGIPZ (shRNA V2‐2)	Thermo Scientific/GE Dharmacon	Cat# RHS4531‐EG51393 cloneID#RHS4430‐200281506‐V3LHS_387624
pMX_GFP‐CAPN2 S50E	Dr. O. DESTAING (IAB, Grenoble, France)
Antibodies
Mouse monoclonal anti‐beta Actin (clone AC‐74)	Sigma‐Aldrich	Cat# A5316, RRID:AB_476743
Rat mnoclonal anti‐CD31 (PECAM‐1) (clone SZ31)	Dianova	Cat# DIA‐310, RRID:AB_2631039
Rabbit monoclonal anti‐Cofilin (clone D3F9) XP®	Cell Signaling Technology	Cat# 5175, RRID:AB_10622000
Rabbit monoclonal anti‐Phospho‐Cofilin (Ser3) (clone 77G2)	Cell Signaling Technology	Cat# 3313, RRID:AB_2080597
Rabbit monoclonal anti‐Phospho‐FAK (Tyr397) (clone D20B1)	Cell Signaling Technology	Cat# 8556, RRID:AB_10891442
Rabbit monoclonal anti‐Phospho‐p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone D13.14.4E) XP®	Cell Signaling Technology	Cat# 4370, RRID:AB_2315112
Mouse monoclonal anti‐Paxillin (clone 349)	BD Biosciences	Cat# 612405, RRID:AB_647289
Rabbit polyclonal anti‐PMEL	Thermo Fisher Scientific	Cat# PA5‐32491, RRID:AB_2549958
Mouse monoclonal anti‐Talin (clone 8d4)	Sigma‐Aldrich	Cat# T3287, RRID:AB_477572
Rabbit polyclonal anti‐TRPV2 (VRL‐1 H‐105)	Santa‐Cruz Biotechnology	Cat # sc‐30155, RRID:AB_2209150
Rabbit polyclonal anti‐TRPV2 (Prestige Antibodies® Powered by Atlas Antibodies)	Sigma‐Aldrich	Cat # HPA044993, RRID:AB_10960889
Mouse monoclonal anti‐Vimentin (clone V9)	Agilent	Cat# M0725, RRID:AB_10013485
Mouse monoclonal anti‐Vinculin (clone VLN01)	Thermo Fisher Scientific	Cat# MA5‐11690, RRID:AB_10976821
Oligonucleotides and sequence‐based reagents
PCR primers	This study and Spinsanti et al (2008)	Appendix Table S1
Chemicals, enzymes and other reagents
SiR‐actin kit	Spirochrome	Cat# SC001
Acti‐Stain 555 Phalloidin	Cytoskeleton	Cat# PHDH1
Rhodamine Phalloidin	Thermo Fisher Scientific	Cat# R415
Fura‐2, AM, cell permeant	Thermo Fisher Scientific	Cat# F1221
(−)‐Cannabidiol	Tocris	Cat# 1570
Tranilast	Sigma‐Aldrich	Cat# T0318
Calpeptin	Selleck Chemicals	Cat# S7396
PD150606	Medchem Express	Cat# HY‐100529
Duolink® In Situ Red Starter Kit Mouse/Rabbit	Sigma‐Aldrich	Cat# DUO92101
Sulfo‐NHS‐LC‐Biotine EZ‐Link™	Thermo Fisher Scientific	Cat# 21335
Pierce™ High Capacity Streptavidin Agarose	Thermo Fisher Scientific	Cat# 20361
Calpain Activity Assay Kit	PromoKine	Cat# PK‐CA577‐K240
Human plasma fibronectin	Sigma‐Aldrich	Cat# F0895
Software
FIJI v1.0	https://fiji.sc	Fiji (RRID:SCR_002285)
PRISM v9.3.1	GraphPAD	GraphPad Prism (RRID:SCR_002798)
MetaFLUOR	Universal imaging	MetaFluor Fluorescence Ratio Imaging Software (RRID:SCR_014294)
RTCA Software	Agilent/ACEA	RTCA Software (RRID:SCR_014821)
Other
EnSpire® 2300 Multilabel Plate Reader	PerkinElmer
xCELLigence RTCA DP System	Agilent/ACEA	Agilent xCELLigence RTCA eSight Real‐Time Cell Analyzer (RTCA) (RRID:SCR_019571)
JuLI™ Stage Real‐Time Cell History Recorder	NanoEnTek Inc
AutoScratch Wound Making Tool	Agilent/BioTek
Gemini twin wave electroporator	BTX