Figure EV2. Inhibition of RET by CPT treatment extends Drosophila lifespan.

• A
  Survival curves comparing control flies and flies fed with food containing 10 μM CPT from day 1 onwards.
• B–D
  Survival curves comparing overall survival (by logrank test) and maximum lifespan (by Wang–Allison test) of control flies and flies fed with food containing 10 μM CPT from 20 days (**P < 0.001 logrank, **P < 0.001 Wang–Allison) (B), 35 days (**P < 0.001 logrank, **P < 0.001 Wang–Allison) (C), and 45 days (ns) (D) onwards (n = 3 groups, 20–25 flies per group).
• E
  Survival curves comparing control flies and flies fed with food containing 40 μM CPT from 35 days onwards (ns; n = 3 groups, 20–25 flies per group).
• F
  Quantification of food consumption in control and CPT‐fed flies (n = 4 groups, 20 per group).

Data information: Data are representative of at least three repeats. Data are shown as mean ± SEM in (F). Survival curves were analyzed with logrank test along with Wang–Allison test for maximum lifespan. Dashed lines mark time points of 50% survival. Asterisks indicate statistical significance (**P < 0.01, ns: not significant) in single‐factor ANOVA with Scheffe's analysis as a post hoc test.