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. 2023 Feb 16;24(4):e55548. doi: 10.15252/embr.202255548

Figure 3. Effect of RET inhibition by CPT on healthspan in Drosophila .

Figure 3

  • A
    Climbing assay of young flies, old flies, and old flies treated with CPT (n = 3 groups, 20 per group).
  • B
    Smurf assay of intestinal integrity in aged flies with or without CPT treatment (n = 3 groups, 20 per group).
  • C
    Images and data quantification showing accumulation of protein aggregates in the brains of aged flies with or without CPT treatment (n = 5).
  • D
    Images and data quantification showing DA neuron mitochondrial morphology in the brains of aged flies with or without CPT treatment (n = 5).
  • E, F
    Images (E) and data quantification (F) showing 4‐HNE levels within or near TH‐positive DA neurons in young flies, old flies, and old flies treated with CPT (n = 4 sets, 5 samples per set). Arrows point to HNE signals co‐labeled with TH‐ positive DA neurons.
  • G
    Quantification of ATP levels in young flies, old flies, and old flies treated with CPT.

Data information: Data are representative of at least three repeats. Data are shown as mean ± SEM. Asterisks indicate statistical significance (**P < 0.01) in single‐factor ANOVA with Scheffe's analysis as a post hoc test.