Figure EV3. Inhibition of RET by genetic manipulation of C‐I subunits extends fly lifespan.

• A, B
  Survival curves of control flies and flies with NDUFS2 (A), or NDUFS3 (B) knocked down in neurons (n = 3 groups, 20–25 flies per group).
• C
  Quantification of food consumption in flies treated with CPT or NMN.
• D
  Quantification of Smurf assay data in young flies, old flies, and old flies treated with CPT and NMN (n = 3 groups, 20 flies per group).
• E–I
  Survival curves showing the lifespan effect of CPT in combination with NMN (E) (control vs NMN: **P < 0.01 logrank, **P < 0.01 Wang Alisson; CPT vs CPT + NMN, ns), mito‐Tempo (F) (control vs mito‐Tempo: **P < 0.01 logrank, **P < 0.01 Wang Alisson; CPT vs CPT + mito‐Tempo: ns), melatonin (G) (control vs melatonin: **P < 0.01 logrank, **P < 0.01 Wang Alisson; CPT vs CPT+ melatonin: ns), FK866 (H) (control vs FK866: **P < 0.01 logrank, **P < 0.01 Wang Alisson; FK866 vs FK866 + CPT: ns), and paraquat (I) (control vs paraquat: **P < 0.01 logrank, **P < 0.01 Wang Alisson; paraquat vs paraquat+CPT: ns) (n = 3 groups, 20 flies per group).

Data information: Data are representative of at least three repeats. Data are shown as mean ± SEM in (C, D). Survival curves were analyzed with logrank test along with Wang–Allison test for maximum lifespan. Dashed lines mark time points of 50% survival. Asterisks indicate statistical significance (**P < 0.01) in single‐factor ANOVA with Scheffe's analysis as a post hoc test.