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. 2023 Feb 6;24(4):e55571. doi: 10.15252/embr.202255571

Figure 4. Requirement of PJA2 for ciliary trafficking.

Figure 4

  1. ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (siPJA2), serum‐deprived for 48 h, and immunostained for acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm. Lower panel shows western blot for PJA2.
  2. Cumulative data of the experiments shown in (A). A mean value ±SD of at least 4 independent experiments is shown. In total 25 cells for each experiment were scored. Student's t test, **P < 0.01.
  3. ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (siPJA2). Cells were serum‐deprived for 48 h and left untreated or stimulated with purmorphamine (puro) for 4 h. Cells were fixed and doubly immunostained for GPR161 (green) and acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm.
  4. Cumulative data of the experiments shown in (C). A mean value ±SD of at least 4 independent experiments is shown. In total, 25 cells for each experiment were scored. Student's t test, **P < 0.01.
  5. ARPE‐19 cells were transiently transfected with control siRNA or siRNA targeting PJA2 (siPJA2). Cells were serum‐deprived for 48 h and left untreated or stimulated with somatostatin (SST) for 3 h. Cells were fixed and doubly immunostained for SSTR3 (green) and acetylated tubulin (red). Nuclei were stained with DAPI (blue). Scale bar, 3 μm.
  6. Cumulative data of the experiments shown in (E). A mean value ±SD of at least 4 independent experiments is shown. In total 25 cells for each experiment were scored. Student's t test, ***P < 0.001.