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. 2023 Feb 2;10(10):2206517. doi: 10.1002/advs.202206517

Figure 2.

Figure 2

EVs‐mediated CasRx delivery in cells. a) Immunostaining with DAPI, CD63, CasRx‐HA, and mCherry in HEK293T cells transfected with the target plasmids. Representative images showed the nuclear localization of CasRx (yellow arrows), colocalization of CD63 and CasRx (white arrowheads), and mCherry‐ CasRx+ cells (white arrows). Scale bar = 50 µm. b) Statistical analysis of the proportion of cells with nuclear localization of CasRx, n = 4 wells per group. c) Statistical analysis of the proportion of cells with colocalization of CD63 and CasRx, n = 4 wells per group. d) Statistical analysis of the proportion of mCherry‐CasRx+ cells, n = 4 wells per group. e) Schematic image (left) and statistical results (right) of CasRx‐HA ELISA. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05. The data are presented as the means ± SD.