Skip to main content
. 2023 Feb 21;24(4):e56524. doi: 10.15252/embr.202256524

Figure 3. The CD106+ population shares pericyte, stem cell and stromal features.

Figure 3

  1. Schematic representation of the experimental setting for PeSC line generation.
  2. PCR for genomic Kras and KrasG12D.
  3. Western Blot analysis for tubulin and mutated KrasG12D proteins in total proteins extracts of PeSC, Epi, and Meta cells.
  4. Representative dot plot of the CD44 and CD24 staining of PeSCs and Epi and Meta cells.
  5. Principal component analysis (PCA) of RNA‐seq data from the indicated cell populations (n = 3).
  6. Pathway enrichment of a list of genes.
  7. qPCR analysis of several genes identified in the RNAseq analysis. Treatment was performed in biological triplicates and qPCR analysis was performed by technical duplicates. The bars are representing the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. The P‐values were calculated using Student's t‐test.
  8. Definition of the PeSC score (bulk RNAseq) applied to the single‐cell RNAseq analysis and identification of cluster 7.
  9. Application of PeSC score to the human PDAC public single‐cell RNAseq database and identification of a cluster with the highest PeSC (red square) among the mesenchymal population (Fig EV2D) in either Normal Pancreas (left) or Human PDAC (right).

Source data are available online for this figure.