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. 2023 Feb 21;24(4):e56524. doi: 10.15252/embr.202256524

Figure EV3. Chimera approach identifies the origin of PeSC.

Figure EV3

  1. Experimental protocol for bone marrow chimeras. One month old KC mice were sublethally irradiated and reconstituted with bone marrow cells from a Tomato expressing mouse. After reconstitution, the chimeras were sacrificed on day 60.
  2. Representative dot plots of reconstituted bone marrow cells (Tomato+) in KC chimeras compared to the nonreconstituted KC mice as control by FACS analysis. Quantification of reconstituted cells (Tomato+) among CD45+ immune cells in either pancreas or periphery of KC chimeras compared to the control. Three mice in each condition.
  3. Representative dot plots and gating strategy of distinguishing the reconstituted CD106+ cells (Tomato+) and nonreconstituted CD106+ cells (Tomato−) in KC chimeras. Three mice in the group, each dot represents one mouse.
  4. Representative IF staining for CD106 (green) and Tomato (red) in the pancreas of chimera mice. Inset shows colocalization of CD106‐ and Tomato‐stained cells. Scale bar, 50 μm (upper), 10 μm (lower).

Data information: ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. The P‐values were calculated using Student's t‐test.