Figure 6. Tumor‐PeSCs crosstalk induces Ly6G⁺ MDSC differentiation.

• A
  Experimental setting of bone marrow differentiation.
• B
  Representative dot plots of CD11b and Ly6G staining in bone marrow cells cultured in the presence of Epi cells, PeSCs or both Epi cells, and PeSCs.
• C
  Quantification of the percentage of Ly6G⁺ cells among CD11b⁺ cells (cumulative from three independent experiments). The bars are representing the mean ± SD.
• D
  Experimental setting for in vivo injection. FACS analysis of the percentages of Ly6G⁺CD45⁺ cells (each dot represents one mouse per group).
• E, F
  Representative immunofluorescence staining of coimplanted Epi cells + PeSCs for Ly6G, mCherry, Nanog, and DAPI. Scale bar, 50 mm.
• G–M
  In vivo depletion of Ly6G⁺ MDSCs diminishes tumor growth. (G) Experimental setting. (H) Tumor weight. FACS analysis of the percentage of CD45⁺ cells (I), and Ly6G⁺ MDSCs (J) derived from the tumor. (K) Representative dot plots of Ly6G‐ and F4/80‐stained CD45⁺ cells. FACS analysis of the percentage of F4/80⁺ cells. (L) Representative dot plots of CD45‐ and CD11c‐stained cells. FACS analysis of the percentage of CD11c⁺ cells among CD45⁺ cells. (M) FACS analysis of GrzB⁺, TNFα⁺, and IFNγ⁺ cells among CD45⁺ cells. Eight mice in each group, five for FACS analysis and three for histological analysis. Each dot represents one mouse.

Data information: *P < 0.05, **P < 0.01, and ***P < 0.001. The P‐values were calculated using Student's t‐test.

Source data are available online for this figure.