Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
Pericyte stem cells	This paper	N/A
Tumoral epithelial cells (Epi)	Goehrig et al (2019)	Goehrig et al (2019)
Lung metastatic cells (Meta)	This paper	N/A
C57BL/6 mouse primary pancreatic microvascular endothelial cells	Cell Biologics	Cat#C57‐6206
C57BL/6 mice (wild type)	Envigo	C57bL/6JOlaHsd
Rag2KO mice	Charles Rivers
P48+/Cre;KrasG12D (KC) mice	Hingorani et al (2003)	See reference
pdx1+/Cre;KrasG12D;p53R172H (KPC) mice	Hingorani et al (2005)	See reference
pdx1+/Cre;KrasG12D;Ink4a/Arffl/fl (KIC) mice	Aguirre et al (2003)	See reference
Recombinant DNA
H2B‐GFP	Addgene	Cat#25999, RRID:Addgene_25999
H2B‐mCherry	Addgene	Cat#20972, RRID:Addgene_20972
Antibodies
Mouse monoclonal anti‐CD24 antibody (clone SN3b)	Invitrogen/Thermo Fisher Scientific	Cat# MA5‐11833, RRID:AB_10985938
Rabbit polyclonal anti‐CD44 antibody	Abcam	Cat# ab157107, RRID:AB_2847859
Rabbit monoclonal recombinant anti‐VCAM1 (CD106) antibody (clone EPR5047)	Abcam	Cat# ab134047, RRID:AB_2721053
Goat polyclonal anti‐alpha smooth muscle actin antibody, N‐term	GeneTex	Cat# GTX89701, RRID:AB_10721877
Rat monoclonal anti‐mouse cytokeratin 19 antibody, unconjugated	DSHB	Cat# TROMA‐III, RRID:AB_2133570
Rabbit monoclonal recombinant anti‐ZO1 tight junction protein antibody (clone EPR19945‐296)	Abcam	Cat# ab221547, RRID:AB_2892660
Rat monoclonal anti‐E‐cadherin antibody (clone ECCD‐2)	Invitrogen/Thermo Fisher Scientific	Cat# 13‐1900, RRID:AB_2533005
Rabbit polyclonal anti‐mCherry antibody	Abcam	Cat# ab167453, RRID:AB_2571870
Goat polyclonal anti‐GFP antibody	Abcam	Cat# ab6673, RRID:AB_305643
Rat monoclonal purified anti‐mouse Ly‐6G antibody (clone 1A8)	BioLegend	Cat# 127602, RRID:AB_1089180
Rabbit polyclonal StemAb™ anti‐mouse Nanog antibody	ReproCell Incorporated	Cat# RCAB002P‐F
Horse anti‐goat IgG antibody (H+L), biotinylated	Vector Laboratories/Eurobio Scientific	Cat# BA‐9500, RRID:AB_2336123
Goat anti‐mouse IgG antibody (H+L), biotinylated	Vector Laboratories/Eurobio Scientific	Cat# BA‐9200, RRID:AB_2336171
Horse anti‐rabbit IgG antibody (H+L), biotinylated	Vector Laboratories/Eurobio Scientific	Cat# BA‐1100, RRID:AB_2336201
Goat anti‐rat IgG antibody, mouse adsorbed (H+L), biotinylated	Vector Laboratories/Eurobio Scientific	Cat# BA‐9401, RRID:AB_2336208
Chicken polyclonal anti‐Rat IgG (H+L) Cross‐Adsorbed Secondary Antibody, Alexa Fluor 647	Invitrogen/Thermo Fisher Scientific	Cat# A‐21472, RRID:AB_2535875
Donkey polyclonal anti‐Rat IgG (H+L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen/Thermo Fisher Scientific	Cat# A‐21208, RRID:AB_2535794
Donkey polyclonal anti‐Rabbit IgG (H+L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 555	Invitrogen/Thermo Fisher Scientific	Cat# A‐31572, RRID:AB_162543
Donkey polyclonal anti‐Rabbit IgG (H+L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen/Thermo Fisher Scientific	Cat# A‐21206, RRID:AB_2535792
Donkey polyclonal anti‐Goat IgG (H+L) Cross‐Adsorbed Secondary Antibody, Alexa Fluor 488	Invitrogen/Thermo Fisher Scientific	Cat# A‐11055, RRID:AB_2534102
Donkey polyclonal anti‐Mouse IgG (H+L) Highly Cross‐Adsorbed Secondary Antibody, Alexa Fluor 555	Invitrogen/Thermo Fisher Scientific	Cat# A‐31570, RRID:AB_2536180
Rat monoclonal anti‐mouse CD45 (clone 30‐F11), APC/Cyanine7 conjugated	BioLegend	Cat# 103116, RRID:AB_312981
Rat monoclonal anti‐mouse CD45 (clone 30‐F11), APC conjugated, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 17‐0451‐82, RRID:AB_469392
Rat monoclonal anti‐mouse CD31 (clone 390), Brilliant Violet 421 conjugated	BioLegend	Cat# 102423, RRID:AB_2562186
Rat monoclonal anti‐mouse/rat CD29 (clone HMβ1‐1), PerCP/Cyanine5.5 conjugated	BioLegend	Cat# 102228, RRID:AB_2572079
Rat monoclonal anti‐mouse CD106 (clone 429 [MVCAM.A]), Alexa Fluor 647 conjugated	BioLegend	Cat# 105712, RRID:AB_493429
Rat monoclonal anti‐mouse CD24 (clone M1/69), PE/Cyanine7 conjugated	BioLegend	Cat# 101822, RRID:AB_756048
Rat monoclonal anti‐mouse/human CD44 (clone IM7), Alexa Fluor 700 conjugated	BioLegend	Cat# 103026, RRID:AB_493713
Rat monoclonal anti‐mouse PDGFRα (clone APA5), Super Bright 600 conjugated, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 63‐1401‐82, RRID:AB_2734880
Rat monoclonal anti‐mouse CD326 (Ep‐CAM) (clone G8.8), Brilliant Violet 510 conjugated	BioLegend	Cat# 118231, RRID:AB_2632774
Rat monoclonal anti‐mouse PDGFRβ (clone APB5), Super Bright 780 conjugated	Invitrogen/Thermo Fisher Scientific	Cat# 78‐1402‐80, RRID:AB_2784899
Armenian hamster monoclonal anti‐mouse CD34 (clone HM34), PE conjugated	BioLegend	Cat# 128610, RRID:AB_2074601
Rat monoclonal anti‐mouse CD90.2 (clone 30‐H12), Brilliant Violet 570 conjugated	BioLegend	Cat# 105329, RRID:AB_10917055
Rat monoclonal anti‐mouse CD105 (clone MJ7/18), Pacific Blue conjugated	BioLegend	Cat# 120412, RRID:AB_2098890
Rat monoclonal anti‐mouse CD146 (clone ME‐9F1), PerCP/Cyanine5.5 conjugated	BioLegend	Cat# 134710, RRID:AB_11203708
Mouse monoclonal anti‐mouse/human/rat αSMA (clone 1A4), eFluor 570 conjugated, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 41‐9760‐82, RRID:AB_2573631
Armenian hamster monoclonal anti‐mouse/rat CD61 (clone 2C9.G2 [HMβ3‐1]), PE/Cyanine7 conjugated	BioLegend	Cat# 104318, RRID:AB_2687361
Rat monoclonal anti‐mouse Ly‐6G (clone 1A8), Brilliant Violet 785 conjugated	BioLegend	Cat# 127645, RRID:AB_2566317
Rat monoclonal anti‐mouse Ly‐6C (clone HK1.4), PE conjugated	BioLegend	Cat# 128008, RRID:AB_1186132
Rat monoclonal anti‐mouse F4/80 (clone BM8), PE/Cyanine7 conjugated	BioLegend	Cat# 123114, RRID:AB_893478
Rat monoclonal anti‐mouse F4/80 (clone BM8), PerCP/Cyanine5.5 conjugated	BioLegend	Cat# 123128, RRID:AB_893484
Armenian hamster monoclonal anti‐mouse CD11c (clone N418), Alexa Fluor 700 conjugated, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 56‐0114‐82, RRID:AB_493992
Armenian hamster monoclonal anti‐mouse CD80 (clone 16‐10A1), Brilliant Violet 605 conjugated	BioLegend	Cat# 104729, RRID:AB_11126141
Rat monoclonal anti‐mouse CD86 (clone GL‐1), APC/Cyanine7 conjugated	BioLegend	Cat# 105030, RRID:AB_2244452
Rat monoclonal anti‐mouse/human CD11b (clone M1/70), Brilliant Violet 421 conjugated	BioLegend	Cat# 101251, RRID: AB_2562904
Human/mouse monoclonal anti‐human/mouse Granzyme B (clone GB11), Alexa Fluor 647 conjugated	BioLegend	Cat#515406, RRID:AB 2566333
Rat monoclonal anti‐mouse Granzyme B (clone 16G6), Biotin, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 13‐8822‐80, RRID:AB_466954
Rat monoclonal anti‐mouse TNF‐α (clone MP6‐XT22), Brilliant Violet 605 conjugated	BioLegend	Cat# 506329, RRID:AB_11123912
Rat monoclonal anti‐mouse IFN‐γ (clone XMG1.2), Brillant Violet 650 conjugated	BioLegend	Cat#505831, RRID:AB_11142685
Rat monoclonal anti‐mouse CD4 (clone RM4‐5), Brilliant Violet 510 conjugated	BioLegend	Cat# 100553, RRID:AB_2561388
Rat monoclonal anti‐mouse CD8a (clone 53‐6.7), Brilliant Violet 421 conjugated	BioLegend	Cat# 100738, RRID:AB_11204079
Rat monoclonal anti‐mouse CD279 (PD‐1) (clone 29F.1A12), PE conjugated	BioLegend	Cat# 135206, RRID:AB_1877231
Rat monoclonal anti‐mouse CD366 (Tim‐3) (clone B8.2C12), APC conjugated	BioLegend	Cat# 134008, RRID:AB_2562998
Rat monoclonal anti‐mouse CD3 (clone 17A2), Brilliant Violet 711 conjugated	BioLegend	Cat# 100241, RRID:AB_2563945
Rat monoclonal anti‐mouse CD107a (LAMP‐1) (clone 1D4B), PE/Cyanine7 conjugated	BioLegend	Cat# 121619, RRID:AB_2562146
Rat monoclonal anti‐mouse CD163 (clone TNKUPJ), PE conjugated, eBioscience	Invitrogen/Thermo Fisher Scientific	Cat# 12‐1631‐82, RRID:AB_2716924
Rat monoclonal anti‐mouse Tim‐4 (clone RMT4‐54), Alexa Fluor 647 conjugated	BioLegend	Cat# 130008, RRID:AB_2271648
Armenian Hamster monoclonal anti‐mouse CD103 (clone 2E7), Brilliant Violet 510 conjugated	BioLegend	Cat# 121423, RRID:AB_2562713
Rat monoclonal anti‐mouse CD335 (NKp46) (clone 29A1.4), Brilliant Violet 421 conjugated	BioLegend	Cat# 137611, RRID:AB_10915472
Rat monoclonal anti‐mouse CD206 (MMR) (clone C068C2), Brilliant Violet 650 conjugated	BioLegend	Cat# 141723, RRID:AB_2562445
Rat monoclonal anti‐mouse CD115 (CSF‐1R) (clone AFS98), PerCP/Cyanine5.5 conjugated	BioLegend	Cat# 135526, RRID:AB_2566462
Rat monoclonal anti‐mouse CD274 (B7‐H1, PD‐L1) (clone 10F.9G2), PerCP/Cyanine5.5 conjugated	BioLegend	Cat# 124334, RRID:AB_2629832
Lectin PNA from arachis hypogaea (peanut), Alexa Fluor 647 conjugate	Invitrogen/Thermo Fisher Scientific	Cat# L32460
InVivoMAb mouse IgG1 isotype control (clone MOPC‐21)	Bio X cell	Cat# BE0083, RRID:AB_1107784
InVivoMab anti‐mouse CD106 (VCAM‐1)	Bio X Cell	Cat# BE0027, RRID:AB_1107572
InVivoPlus anti‐mouse PD‐1 (CD279)	Bio X Cell	Cat# BE0146, RRID:AB_10949053
InVivoPlus anti‐mouse Ly6G antibody	Bio X Cell	Cat# BE0075‐1, RRID:AB_1107721
Mouse IgG2A isotype control (clone 20102)	R&D systems	Cat# MAB003, RRID:AB_357345
Mouse CCL5/RANTES Antibody	R&D Systems	Cat# MAB478, RRID:AB_2290968
Ras (G12D Mutant Specific) (D8H7) Rabbit mAb	Cell Signaling	Cat# 14429S, RRID:AB_2728748
Peroxidase‐AffiniPure donkey anti‐rabbit IgG (H+L) antibody	Jackson ImmunoResearch	Cat# 711‐035‐152, RRID:AB_10015282
Oligonucleotides and other sequence‐based reagents
Primers for Nanog: Forward: 5′‐TCTCTCAGGCCCAGCTGTGT‐3′ and Reverse: 5′‐GCTTGCACTTCATCCTTTG‐3′	Intergrated DNA Technologies	N/A
Primers for Oct3/4: Forward: 5′‐GCCCTGCAGAAGGAGCTAGAAC‐3′ and Reverse: 5′‐GGAATACTCAATACTTGATCT‐3′	Intergrated DNA Technologies	N/A
Primers for Nkx3.2: Forward: 5′‐AGATGTCAGCCAGCGTTTC‐3′ and Reverse: 5′‐AGGGCTAACGCTGTCATCCT‐3′	Intergrated DNA Technologies	N/A
Primers for RGS: Forward: 5′‐GCTTTGACTTGGCCCAGAAA‐3′ and Reverse: 5′‐CCTGACCAGATGACTACTTGATTAGCT‐3′	Intergrated DNA Technologies	N/A
Primers for PDGFRα: Forward: 5′‐TCTGTGACTTTTAAGGATGCTTCA‐3′ and Reverse: 5′‐GATGCCCACATAGCCTTCATTC‐3′	Intergrated DNA Technologies	N/A
Primers for PDGFRβ: Forward: 5′‐TCATGAAGCCAGCAAGAGTG‐3′ and Reverse: 5′‐GTGGTAATCCCGTCAGCATC‐3′	Intergrated DNA Technologies	N/A
Primers for ACTA2: Forward: 5′‐GCCAGTCGCTGTCAGGAACCC‐3′ and Reverse: 5′‐AGCCGGCCTTACAGAGCCCA‐3′	Intergrated DNA Technologies	N/A
Primers for Prominin1: Forward: 5′‐GCCCAAGCTGGAAGAATATG‐3′ and Reverse: 5′‐CAGCAGAAAGCAGACAATCAA‐3′	Intergrated DNA Technologies	N/A
Primers for Snail1: Forward: 5′‐CTTGTGTCTGCACGACCTGT‐3′ and Reverse: 5′‐CAGGAGAATGGCTTCTCACC‐3′	Intergrated DNA Technologies	N/A
Primers for Snail2: Forward: 5′‐CATTGCCTTGTGTCTGCAAG‐3′ and Reverse: 5′‐AGAAAGGCTTTTCCCCAGTG‐3′	Intergrated DNA Technologies	N/A
Primers for Sparc: Forward: 5′‐AGAGGAAACGGTCGAGGAG‐3′ and Reverse: 5′‐CTCACACACCTTGCCATGTT‐3′	Intergrated DNA Technologies	N/A
Primers for Twist: Forward: 5′‐AGCTACGCCTTCTCCGTCT‐3′ and Reverse: 5′‐TCCTTCTCTGGAAACAATGACA‐3′	Intergrated DNA Technologies	N/A
Primers for Zeb2: Forward: 5′‐CCAGAGGAAACAAGGATTTCAG‐3′ and Reverse: 5′‐AGGCCTGACATGTAGTCTTGTG‐3′	Intergrated DNA Technologies	N/A
Primers for Zeb1: Forward: 5′‐TGAGCACACAGGTAAGAGGCC‐3′ and Reverse: 5′‐GGCTTTTCCCCAGAGTGCA‐3′	Intergrated DNA Technologies	N/A
Primers for Col6a: Forward: 5′‐GCAAGGATGAGCTGGTCAA‐3′ and Reverse: 5′‐GTCCACGTGCTCTTGCATC‐3′	Intergrated DNA Technologies	N/A
Primers for Epcam: Forward: 5′‐GATTCTGCACGTGAGACCTG‐3′ and Reverse: 5′‐GATACCAAGTCAAACCGAGAACTT‐3′	Intergrated DNA Technologies	N/A
Primers for Fn1: Forward: 5′‐CGGAGAGAGTGCCCCTACTA‐3′ and Reverse: 5′‐CGATATTGGTGAATCGCAGA‐3′	Intergrated DNA Technologies	N/A
Primers for Krt19: Forward: 5′‐TCCCAGCTCAGCATGAAAGCT‐3′ and Reverse: 5′‐AAAACCGCTGATCACGCTCTG‐3′	Intergrated DNA Technologies	N/A
Primers for Krt7: Forward: 5′‐CACGAACAAGGTGGAGTTGGA‐3′ and Reverse: 5′‐TGTCTGAGATCTGCGACTGCA‐3′	Intergrated DNA Technologies	N/A
Primers for Sox9: Forward: 5′‐CAAGACTCTGGGCAAGCTCTG‐3′ and Reverse: 5′‐TCCGCTTGTCCGTTCTTCAC‐3′	Intergrated DNA Technologies	N/A
Primers for GAPDH: Forward: 5′‐CAACGACCCCTTCATTGACC‐3′ and Reverse: 5′‐GGTCTCGCTCCTGGAAGATG‐3′	Intergrated DNA Technologies	N/A
PrimePCR™ SYBR® Green Assay CD248 for Mouse	Bio‐Rad	Cat#10025636
KrasG12D conditional primers for PCR: con1: 5′‐GTC TTTCCCCAGCACAGTGC‐3′; con2: 5′‐CTCTTGCCTACGCCACCAGCT C‐3′; con3: 5′‐AGCTAGCCACCATGGCTTGAGTAAGTCTGCA‐3′	Intergrated DNA Technologies	https://jacks‐lab.mit.edu/protocols/genotyping/kras_cond
Chemicals, Enzymes and other reagents
Complete mouse endothelial cell medium	Cell Biologics	Cat# M1168
Phosphate‐buffered saline (PBS) 10×	Gibco/Thermo Fisher Scientific	Cat# 14190‐144
Dulbecco's Modified Eagle Medium (DMEM)	Gibco/Thermo Fisher Scientific	Cat# 61965059
Fetal calf serum (FCS)	Eurobio Scientific	Cat# CVFSVF00‐01
Penicillin‐Streptomycin (P/S)	Gibco/Thermo Fisher Scientific	Cat# 15140‐122
Collagenase P	Roche/Sigma‐Aldrich	Cat# 11213865001
16% Formaldehyde solution (PFA)	Termo Fisher	Cat# 28908
10% Neutral buffered formalin	Diapath S.p.A.	REF: F0043
Triton X‐100	Sigma‐Aldrich	Cat# T8787
Antigen Unmasking Solution, Citrate‐Based	Vector Laboratories/Eurobio Scientific	Cat# H‐3300
DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'‐diaminobenzidine)	Vector Laboratories/Eurobio Scientific	Cat# SK‐4100
Mayer Hematoxylin	Diapath S.p.A.	REF: C0303
Antibody diluent Reagent	Life Techno/Thermo Fisher	Cat# 003218
Vectashield mounting medium with DAPI	Vector Laboratories/Eurobio Scientific	Cat# H‐1200
NuPAGE 4‐12% Bis‐Tris polyacrylamide gel	Invitrogen/Thermo fischer	Cat# NP0335BOX
Immune‐Blot PVDF membrane	BIO‐RAD	Cat# 1620177
Tris buffered saline (TBS)	Euromedex	Cat# ET220
Tween 20	VWR	Cat# 28829.296
Bovine serum albumin (BSA)	Sigma	Cat# A2153‐100G
RIPA buffer	Pierce/Thermo Fisher	Cat# 89900
Protease complete EDTA‐free	Roche/Sigma‐Aldrich	Cat# 04 693 159 001
Phosphatase phoSTOP	Roche/Sigma‐Aldrich	Cat# 04 906 837 001
Cell Activation Cocktail (w/o Brefeldin A)	BioLegend	Cat# 423301
Brefeldin A solution	BioLegend	Cat# 420601
Corning® Matrigel® Basement Membrane Matrix High Concentration	Corning	Cat# 354248
Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red‐free, LDEV‐free	Corning	Cat# 356231
Recombinant Murine PDGF‐BB	PeproTech/Thermo Fisher Scientific	Cat# 315‐18
TGF‐β1	Biotechne	Cat#7666‐MB
IncuCyte® Zoom live‐cell analysis system	Essen Bioscience
Other
PA485: Pancreatitis and matched pancreatic cancer tissue array	US Bioomax, Inc.	https://www.biomax.us/tissue‐arrays/pancreas/PA485
BIC14011a: Pancreas intraepithelial neoplasia, pancreatitis and cancer tissue array	US Bioomax, Inc.	https://www.biomax.us/tissue‐arrays/pancreas/BIC14011a
StemPro® Osteogenesis Differentiation Kit	Gibco/Thermo Fisher Scientific	Cat#10072‐01
StemPro® Chondrogenesis Differentiation Kit	Gibco/Thermo Fisher Scientific	Cat#10071‐01
Alizarin Red S Staining Kit	ScienCell Research Laboratories	Cat#0223
Alcian Blue 8GX	Sigma‐Aldrich	Cat#A3157‐10G
True‐Nuclear™ Transcription Factor Buffer Set	BioLegend	Cat#424401
NucleoSpin® RNA Plus kit	MACHEREY‐NAGEL	REF 740984
CellTrace™ CFSE Cell Proliferation Kit	Invitrogen/Thermo Fisher Scientific	Cat#C34554
LEGENDplex™ Custom Mouse 5‐plex Panel	BioLegend	Cat# B285861
ECL Western Blotting Substrate	Pierce/Thermo Fisher Scientific	Cat# 32106