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. 2023 Mar 14;26(4):106399. doi: 10.1016/j.isci.2023.106399

Figure 1.

Figure 1

GFP to BFP conversion hiPSC model to study CRISPR/Cas9 genome editing and KI efficiency by flow cytometry

(A) Schematic representation of GFP to BFP conversion strategy using CRISPR/Cas9 and a BFP ssODN. PAM is written in bold, KI in blue and DraIII restriction site is underlined.

(B) Phenotyping strategy used to determine KI (BFP expressing cells) and KO (non-fluorescent cells) rates. The figures were created with https://www.biorender.com/ (Toronto, Canada). GFP, green fluorescent protein; BFP, blue fluorescent protein; dgRNA, dual guide RNA; ssODN, single-stranded oligonucleotide; hiPSCs, induced pluripotent stem cells; KI, knockin; KO, knockout.